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Expression of Multiple Functional RNAs or Proteins from One Viral Vector.

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Author

Summary, in English

In this chapter, we will cover the available design choices for enabling expression of two functional protein or RNA sequences from a single viral vector. Such vectors are very useful in the neuroscience-related field of neuronal control and modulation, e.g., using optogenetics or DREADDs, but are also desirable in applications of CRISPR/Cas9 in situ genome editing and more refined therapeutic approaches. Each approach to achieving this combined expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.

Publishing year

2016

Language

English

Pages

41-56

Publication/Series

Methods in Molecular Biology

Volume

1382

Document type

Journal article

Publisher

Springer

Topic

  • Cell and Molecular Biology

Status

Published

Research group

  • Molecular Neuromodulation

ISBN/ISSN/Other

  • ISSN: 1940-6029