Lactate dehydrogenase activity and isoform distribution in normal and hypertrophic smooth muscle tissue from the rat
Author
Summary, in English
The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal vein and urinary bladder. In the hypertrophic urinary bladder a shift in LDH isoform pattern towards less of the H form was found, which correlates with a better maintenance of contraction in anoxia in this type of hypertrophic smooth muscle.
Publishing year
1991
Language
English
Pages
230-234
Publication/Series
Pflügers Archiv
Volume
419
Issue
3-4
Document type
Journal article
Publisher
Springer
Topic
- Physiology
Keywords
- Lactate dehydrohgenase
- Smooth muscle
- Isoforms
- Hypertrophy
- Rat urinary bladder
- Rat portal vein
- Rat arota
Status
Published
Research group
- Vascular Physiology
- Urology
ISBN/ISSN/Other
- ISSN: 0031-6768