Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis
Author
Summary, in English
Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.
Department/s
- Department of Immunotechnology
- Computational Biology and Biological Physics - Has been reorganised
- Department of Translational Medicine
- Division of Translational Cancer Research
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2011
Language
English
Pages
1645-1656
Publication/Series
Journal of Proteome Research
Volume
10
Issue
4
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Biophysics
- Immunology in the medical area
Keywords
- isotopic labeling
- HPLC-MS
- 2D-PAGE
- plasma membrane
- protein
- expression
- mRNA expression
- correlation
- hypoxia
- normoxia
- neuroblastoma
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-3893