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Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.

På svenska

Author

  • Jana Braesel
  • Sebastian Götze
  • Firoz Shah
  • Daniel Heine
  • James Tauber
  • Christian Hertweck
  • Anders Tunlid
  • Pierre Stallforth
  • Dirk Hoffmeister

Summary, in English

The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.

Publishing year

2015

Language

English

Pages

1325-1334

Publication/Series

Chemistry and Biology

Volume

22

Issue

10

Document type

Journal article

Publisher

Cell Press

Topic

  • Microbiology
  • Forest Science

Status

Published

Project

  • MICCS - Molecular Interactions Controlling soil Carbon Sequestration

Research group

  • Microbial Ecology

ISBN/ISSN/Other

  • ISSN: 1879-1301