The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Characterization of Lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern Sweden

På svenska

Author

  • Katharina Ornstein
  • Johan Berglund
  • Ingrid Nilsson
  • Ragnar Norrby
  • Sven Bergström

Summary, in English

Southern Sweden is an area of Lyme borreliosis (LB) endemicity, with an incidence of 9 cases per 100,000 inhabitants. The most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). There was no record of human Borrelia strains being isolated front patients in this region before the prospective study reported here. Borrelia spirochetes were isolated from skin and cerebrospinal fluid (CSF) from LB patients living in the region. A total of 39 strains were characterized by OspA serotype analysis, species-specific PCR, and signature nucleotide analysis of the 16S rRNA gene. Of 33 skin isolates, 31 (93.9%) were Borrelia afzelii strains and 2 (6.1%) were Borrelia garinii strains. Of six CSF isolates, five (83,3%) were B. garinii and one (16.7%) was B. afzelii, Neither Borrelia burgdorferi sensu stricto strains nor multiple infections were observed, The B. afzelii isolates were of OspA serotype 2, Three B, garinii strains were of OspA serotype 5, and the remaining four strains were of OspA serotype 6, All of the B, garinii strains belonged to the same 16S ribosomal DNA ribotype class, Our findings agree with earlier findings front other geographic regions in Europe where B. afzelii and B, garinii have been recovered predominately from skin and CSF cultures, respectively, To further study the possible presence in Sweden of the genotype B. burgdorferi sensu stricto, which is known to be present in Europe and to occur predominately in patients with Lyme arthritis, molecular detection of Borrelia-specific DNA in synovial samples from LS me arthritis patients should he performed.

Publishing year

2001

Language

English

Pages

1294-1298

Publication/Series

Journal of Clinical Microbiology

Volume

39

Issue

4

Document type

Journal article

Publisher

American Society for Microbiology

Topic

  • Microbiology in the medical area

Status

Published

Research group

  • Family Medicine and Community Medicine

ISBN/ISSN/Other

  • ISSN: 1098-660X