The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Tracing Changes in the Microbial Community of a Hydrocarbon-Polluted Soil by Culture-Dependent Proteomics

På svenska

Author

  • F. Bastida
  • César Nicolás Cuevas
  • J. L. Moreno
  • T. Hernandez
  • C. Garcia

Summary, in English

Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani (LB) media showed a change in the microbial community. The majority of the proteins were related to glycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.

Publishing year

2010

Language

English

Pages

479-485

Publication/Series

Pedosphere

Volume

20

Document type

Journal article

Publisher

Institute of Soil Science

Topic

  • Biological Sciences

Keywords

  • culture dependent
  • hydrocarbon contamination
  • microbial diversity
  • proteomics
  • semiarid soil
  • symbiobacterium-thermophilum
  • bacterial diversity
  • disturbance
  • biomass
  • impact
  • growth

Status

Published

ISBN/ISSN/Other

  • ISSN: 1002-0160