Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.

Summary, in English

The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.

• Author: Karol Bal; Lennart Larsson; Eugenia Mielniczuk; Zbigniew Mielniczuk
• Department/s: Division of Medical Microbiology
• Publishing year: 2002
• Language: English
• Pages: 267-270
• Publication/Series: Journal of Microbiological Methods
• Volume: 48
• Issue: 2-3
• Links: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11777574&dopt=Abstract; http://dx.doi.org/10.1016/S0167-7012(01)00328-1
• Document type: Journal article
• Publisher: Elsevier
• Topic: Microbiology in the medical area
• Keywords: Muramic Acids : chemistry; Trimethylsilyl Compounds : chemistry; Spectrum Analysis Mass; Peptidoglycan : analysis; Bacteria : chemistry
• Status: Published
• ISBN/ISSN/Other: ISSN: 1872-8359