Corticotropin releasing factor-Distribution in rat intestine and role in neuroprotection
Author
Summary, in English
Aims of the present stud were to describe the distribution of corticotropin releasing factor (CRF) immunoreactivity in rat small and large intestines, to quantify the percentage of CRF-immunoreactive (CRF-IR) enteric neurons, to reveal possible CRF immunoreactivity in cultured myenteric neurons from rat ileum and to examine if additions of CRF, urocortin 1 (Ucn1), CRF antagonist or vasoactive intestinal peptide (VIP) affect neuronal survival in vitro. Co-localization of CRF- and VIP-immunoreactivity was examined, as well as a possible interplay between CRF and VIP in neuroprotection. Further we wanted to elucidate if mast cells affect neuronal survival via CRF signaling. Networks of CRF-containing nerve cell bodies and fibers were detected in rat intestine. CRF-IR neurons contained to a high degree also VIP. A low number of cultured myenteric neurons was CRF-IR. CRF, Ucn1 or CRF-antagonist did not promote neuronal survival of cultured myenteric neurons, while VIP significantly enhanced neuronal survival. Simultaneous presence of CRF attenuated the VIP mediated increase in neuronal survival. Co-culturing neurons and mast cells resulted in a marked reduction in neuronal survival, not executed via CRF signaling pathways. Conclusion: CRF is present in enteric neurons and counteracts the neuroprotective effect of VIP in vitro. (c) 2010 Elsevier B.V. All rights reserved.
Department/s
- Neurogastroenterology
Publishing year
2011
Language
English
Pages
68-75
Publication/Series
Regulatory Peptides
Volume
166
Issue
1-3
Document type
Journal article
Publisher
Elsevier
Topic
- Cell and Molecular Biology
Keywords
- CRF
- Mast cells
- Myenteric neurons
- Primary culture
- VIP
Status
Published
Research group
- Neurogastroenterology
ISBN/ISSN/Other
- ISSN: 1873-1686