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Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes.

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Author

  • Oktar Guloglu
  • Anna Larsen

Summary, in English

Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson's disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for the dopaminergic induction of mouse embryonic stem cells and NTERA2 cells as well.

Publishing year

2016

Language

English

Pages

235-244

Publication/Series

Methods in Molecular Biology

Volume

1341

Document type

Journal article

Publisher

Springer

Topic

  • Basic Medicine

Status

Published

ISBN/ISSN/Other

  • ISSN: 1940-6029