Calmidazolium inhibits muscarinic receptor-mediated PLC activation in SH-SY5Y cells
Author
Summary, in English
The aim of this study was to investigate the effect of a calmodulin antagonist, calmidazolium, on the muscarinic receptor-mediated increase in inositol 1,4,5-trisphosphate [I(1,4,5)P3] in SH-SY5Y cells. Exposure to 10 microM calmidazolium suppressed the initial I(1,4,5)P3 peak increase (IC50 1 microM) whereas the steady-state was less affected. Furthermore, calmidazolium displayed non-competitive antagonistic properties of [3H]quinuclidinyl benzylate binding to intact SH-SY5Y cells and to membranes from these cells. These effects were also obtained with another calmodulin inhibitor, trifluoperazine (10 microM). These results demonstrate that novel finding that the calmodulin inhibitors calmidazolium and trifluoperazine act as non-competitive muscarinic antagonists in SH-SY5Y cells and inhibit muscarinic receptor-stimulated phospholipase C activation in these cells.
Publishing year
1995
Language
English
Pages
1333-1337
Publication/Series
NeuroReport
Volume
6
Issue
9
Document type
Journal article
Publisher
Lippincott Williams & Wilkins
Topic
- Neurosciences
Status
Published
ISBN/ISSN/Other
- ISSN: 1473-558X