Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets
Author
Summary, in English
The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.
Department/s
Publishing year
1989
Language
English
Pages
169-174
Publication/Series
Drug and Alcohol Dependence
Volume
24
Issue
2
Document type
Journal article
Publisher
Elsevier
Topic
- Substance Abuse
Keywords
- ethanol
- phospholipase C
- serotonin
- phosphatidic acid
- platelets
Status
Published
Research group
- Clinical Chemistry, Malmö
- Experimental Epilepsy Group
ISBN/ISSN/Other
- ISSN: 1879-0046