New perspectives on pathology in Huntington's disease - characterization of hypothalamic changes
Author
Summary, in English
In the Paper I and II included in this thesis, we quantified selective neuropeptide-ex- pressing hypothalamic populations known to regulate metabolism and emotion in patients with HD compared to healthy controls. We showed that both oxytocin- and vasopressin- expressing neurons were decreased in HD cases. These reductions were suggested to ap- pear at an early disease stage before striatal pathology has occurred. Also in the first study, we demonstrated that the number of cocaine- and amphetamine-regulated transcript (CART)-expressing neurons was increased in HD cases. We also demonstrated that the both oxytocin- and vasopressin-expressing neurons reductions were accompanied with cell loss in the paraventricular nucleus in the hypothalamus of HD cases.
In study three, we developed a robust method to measure the hypothalamic volume in magnetic resonance imaging (MRI) in order to determine whether the hypothalamic dysfunction in HD is associated with the volume of this region. We performed hypotha- lamic volumetric analyses on prodromal HD, symptomatic HD and control participants who underwent MRI scanning at baseline and 18 months follow-up as a part of the IM- AGE-HD study. We found no evidence of cross-sectional or longitudinal changes between groups in hypothalamic volume, suggesting that hypothalamic pathology in HD is not associated with volume changes.
In our forth study, we investigated whether transcriptional dysfunction in emotion and metabolism regulating factors in hypothalamic nuclei were present in HD. We found that an array of genes involved in the neuropeptide expression, autophagy, neurotropic factors, neurogenesis and receptor signaling were altered in selective hypothalamic nuclei in the HD cases.
Taken together, the results from this thesis demonstrate that hypothalamic dysfunction is a part of HD pathology, which could contribute to the pathogenesis of several non- motor symptoms of the disease. These findings might have the potential to give rise to new and more effective therapeutic interventions.
Department/s
Publishing year
2015
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2015:47
Document type
Dissertation
Publisher
Translational Neuroendocrinology
Topic
- Neurosciences
Keywords
- Huntington’s disease
- huntingtin
- hypothalamus
- oxytocin
- vasopressin
- orexin
- CART
- neuroendocrinology
- MRI
- 3 Tesla
- volymetric analysis
Status
Published
Research group
- Translational Neuroendocrinology
Supervisor
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-7619-126-2
Defence date
13 May 2015
Defence time
09:00
Defence place
Belfragsalen, BMC D15, Klinikgatan 32, Lund
Opponent
- Roger Barker