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Regulation of phospholipase C-gamma 2 via phosphatidylinositol 3-kinase in macrophages.

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Author

  • Gösta Hiller
  • Roger Sundler

Summary, in English

Phospholipase C-gamma (PLC-gamma) isoforms are thought to be activated by both tyrosine phosphorylation and phosphatidylinositol 3,4,5 trisphosphate (PtdIns 3,4,5 P(3)), the product of phosphatidylinositol 3-kinase (PtdIns 3-kinase). In this study, we show that stimulation of mouse macrophages with either zymosan beads or bacteria (Prevotella intermedia) induced tyrosine phosphorylation of PLC-gamma 2. Zymosan stimulation also induced translocation to membrane and cytoskeleton fractions, which was inhibited by the PtdIns 3-kinase inhibitors wortmannin and LY 294002. However, the tyrosine phosphorylation of PLC-gamma 2 induced by zymosan was not affected by the inhibitors wortmannin and LY 294002. In contrast to zymosan and bacteria, PLC-gamma 2 was not phosphorylated by stimulation with lipopolysaccharide (LPS), phorbol ester or calcium ionophore. Moreover, the PLC-gamma 1 isoform was not detected in mouse macrophages. These data indicate that PtdIns 3-kinase is critical for the translocation but not for the tyrosine phosphorylation of PLC-gamma 2 in mouse macrophages and that the latter may be insufficient for enzyme activation.

Publishing year

2002

Language

English

Pages

169-173

Publication/Series

Cellular Signalling

Volume

14

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Microbiology

Keywords

  • Chromones : pharmacology
  • Cytoskeleton : enzymology
  • Dose-Response Relationship Drug
  • Female
  • Macrophages : drug effects : enzymology
  • Isoenzymes : metabolism
  • Mice
  • Morpholines : pharmacology
  • Phosphotyrosine : metabolism
  • Phosphorylation
  • Phospholipase C : metabolism
  • Prevotella intermedia
  • Protein Transport
  • Signal Transduction
  • Support Non-U.S. Gov't
  • Zymosan : pharmacology
  • Cells Cultured
  • Cell Membrane : enzymology
  • Animal
  • Androstadienes : pharmacology
  • 1-Phosphatidylinositol 3-Kinase : antagonists & inhibitors : physiology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-3913