Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements

Summary, in English

This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.

• Author: T Kanno; Xiaosong Ma; Sebastian Barg; Lena Eliasson; Juris Galvanovskis; Sven Göpel; Max Larsson; Erik Renström; Patrik Rorsman
• Department/s: Department of Experimental Medical Science; Islet cell physiology; Diabetes - Islet Cell Exocytosis; Neurophysiology; Diabetes - Islet Patophysiology
• Publishing year: 2004
• Language: English
• Pages: 302-311
• Publication/Series: Methods
• Volume: 33
• Issue: 4
• Links: http://dx.doi.org/10.1016/j.ymeth.2004.01.003
• Document type: Journal article
• Publisher: Elsevier
• Topic: Endocrinology and Diabetes
• Status: Published
• Research group: Islet cell physiology; Diabetes - Islet Cell Exocytosis; Neurophysiology; Diabetes - Islet Patophysiology
• ISBN/ISSN/Other: ISSN: 1095-9130