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Decreased Gene Expression of Fibrillin-1 in Stress Urinary Incontinence

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Author

  • Marie Westergren Soderberg
  • Birgitta Bystrom
  • Margareta Hammarstrom
  • Anders Malmström
  • Gunvor Ekman-Ordeberg

Summary, in English

Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA expression by real-time RTPCR and protein localization by immunohistochemistry were analyzed concerning collagen I and III, the small leucine rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin and the elastic fiber associated proteins fibulin-5 and fibrillin-1. Statistical comparisons controlled for age changes in gene expressions. Results: A significant decrease in mRNA expression of fibrillin-1 was discovered in all SUI women compared to all controls, P = 0.03. All molecules were down-regulated by age, but no other differences between SUI and controls reached significance. All proteins were adequately expressed by immunohistochemistry. A weaker staining for fibrillin-1 was seen in the pre-menopausal SUI group compared to the pre-menopausal controls. Conclusions: A decreased gene signal and weaker immunoreactivity for fibrillin-1, important for the elastic fiber assembly, was discovered in women with SUI. Loss of tissue elasticity could lead to increased urethra hypermobility and SUI. Neurourol. Urodynam. 29:476-481, 2010. (C) 2009 Wiley-Liss, Inc.

Department/s

  • Matrix Biology

Publishing year

2010

Language

English

Pages

476-481

Publication/Series

Neurourology and Urodynamics

Volume

29

Issue

3

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Urology and Nephrology

Keywords

  • proteoglycan
  • incontinence
  • extracellular matrix
  • collagen
  • elastin

Status

Published

Research group

  • Matrix Biology

ISBN/ISSN/Other

  • ISSN: 0733-2467