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An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

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Author

Summary, in English

Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu mol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol ( r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.

Publishing year

2007

Language

English

Pages

1772-1780

Publication/Series

Journal of Lipid Research

Volume

48

Issue

8

Document type

Journal article

Publisher

American Society for Biochemistry and Molecular Biology

Topic

  • Medicinal Chemistry

Keywords

  • enzyme-linked immunosorbent assay
  • interval project
  • lipocalin
  • nordic reference
  • and database
  • reference interval project bio-bank
  • nordic
  • biobank
  • reference range
  • reference interval
  • high density lipoprotein
  • low density lipoprotein

Status

Published

Research group

  • Clinical Chemistry, Malmö

ISBN/ISSN/Other

  • ISSN: 1539-7262