Two separate UV-B radiation wavelength regions control expression of different molecular markers in Arabidopsis thaliana
Author
Summary, in English
Fluence-response curves were obtained at nine wavelengths in the interval 280--360 nmformRNAtranscripts of
four molecular markers induced by ultraviolet-B (UV-B) radiation in Arabidopsis thaliana (L.) Heynh.: CHS (encoding chalcone synthase), PDX1.3 (encoding an enzyme involved in formation of pyridoxine), MEB5.2 (encoding a protein with unknown function but which is strongly upregulated by UV-B), and LHCB1*3 (encoding a chlorophyll a/b binding protein). Intact Arabidopsis plants were irradiated for 3 h using a high intensity deuterium radiation source and narrow bandwith filters without supplementary PAR. The results obtained suggest the existence of two distinct UV-B signal responses: one sensitive between 300 and 310 nm and the other sensitive around 280--290 nm. Among the investigated molecular markers, CHS and PDX1.3 were regulated through the chromophore absorbing around 300 nm, whereas MEB5.2 and LHCB1*3 were regulated through the chromophore absorbing at 280--290 nm. The results obtained show that at least two signal transduction pathways exist that regulate gene expression as a result of absorption of UV-B radiation in plants.
four molecular markers induced by ultraviolet-B (UV-B) radiation in Arabidopsis thaliana (L.) Heynh.: CHS (encoding chalcone synthase), PDX1.3 (encoding an enzyme involved in formation of pyridoxine), MEB5.2 (encoding a protein with unknown function but which is strongly upregulated by UV-B), and LHCB1*3 (encoding a chlorophyll a/b binding protein). Intact Arabidopsis plants were irradiated for 3 h using a high intensity deuterium radiation source and narrow bandwith filters without supplementary PAR. The results obtained suggest the existence of two distinct UV-B signal responses: one sensitive between 300 and 310 nm and the other sensitive around 280--290 nm. Among the investigated molecular markers, CHS and PDX1.3 were regulated through the chromophore absorbing around 300 nm, whereas MEB5.2 and LHCB1*3 were regulated through the chromophore absorbing at 280--290 nm. The results obtained show that at least two signal transduction pathways exist that regulate gene expression as a result of absorption of UV-B radiation in plants.
Department/s
Publishing year
2008
Language
English
Pages
222-227
Publication/Series
Functional Plant Biology
Volume
35
Issue
3
Document type
Journal article
Publisher
CSIRO Publishing
Topic
- Biological Sciences
Keywords
- photoreceptor UV-B irradiation wavelength dependence
Status
Published
ISBN/ISSN/Other
- ISSN: 1445-4408