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Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.

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Author

  • Emilia Heimann
  • Helena Jones
  • Svante Resjö
  • Vincent C Manganiello
  • Lena Stenson
  • Eva Degerman

Summary, in English

As shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme. Here, we show that PDE3B is activated in response to high glucose, insulin and cAMP elevation in rat pancreatic islets and INS-1 (832/13) cells. Activation by glucose was not affected by the presence of diazoxide. PDE3B activation was coupled to an increase as well as a decrease in total phosphorylation of the enzyme. In addition to PDE3B, several other PDEs were detected in human pancreatic islets: PDE1, PDE3, PDE4C, PDE7A, PDE8A and PDE10A. We conclude that PDE3B is activated in response to agents relevant for β-cell function and that activation is linked to increased as well as decreased phosphorylation of the enzyme. Moreover, we conclude that several PDEs are present in human pancreatic islets.

Publishing year

2010

Language

English

Publication/Series

PLoS ONE

Volume

5

Issue

12

Document type

Journal article

Publisher

Public Library of Science (PLoS)

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Insulin Signal Transduction
  • Molecular Endocrinology

ISBN/ISSN/Other

  • ISSN: 1932-6203