The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo

På svenska

Author

  • Aras Kadioglu
  • Jason A. Sharpe
  • Irene Lazou
  • Catharina Svanborg
  • Colin Ockleford
  • Tim J. Mitchell
  • Peter W. Andrew

Summary, in English

The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.

Publishing year

2001

Language

English

Pages

105-110

Publication/Series

FEMS Microbiology Letters

Volume

194

Issue

1

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Microbiology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1574-6968