Detection of Helicobacter rodentium-like DNA in the liver tissue of patients with chronic liver diseases by polymerase chain reaction-denaturing gradient gel electrophoresis and DNA sequence analysis

Summary, in English

Many Helicobacter spp. were isolated from the stomach, intestinal tract, and liver of different animals and humans. The association between Helicobacter spp. and hepatobiliary diseases, including hepatocellular carcinoma, was thoroughly examined, indicating a potential role of the bacteria in the progression toward cancer. In our work, we screened 97 liver biopsies from patients with chronic liver diseases for the presence of Helicobacter spp. DNA. With the use of genus-specific polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing, we found that the majority of Helicobacter spp. DNA detected was similar to Helicobacter rodentium DNA (71%). The DNA of other detected Helicobacter spp. was similar to Helicobacter pylori DNA. This is the first indication of H. rodentium-like DNA presence in human liver tissue. We also conclude that PCR DGGE is a useful screening method for assigning species designation and heterogeneity. (C) 2010 Elsevier Inc. All rights reserved.

• Author: Joanna Nakonieczna; Piotr Stalke; Waleed Abu Al-Soud; Torkel Wadström; Krzysztof Piotr Bielawski
• Department/s: Division of Medical Microbiology
• Publishing year: 2010
• Language: English
• Pages: 201-207
• Publication/Series: Diagnostic Microbiology and Infectious Disease
• Volume: 68
• Issue: 3
• Links: http://dx.doi.org/10.1016/j.diagmicrobio.2010.07.001
• Document type: Journal article
• Publisher: Elsevier
• Topic: Microbiology in the medical area
• Keywords: PCR-DGGE; Helicobacter; Chronic liver diseases
• Status: Published
• ISBN/ISSN/Other: ISSN: 1879-0070