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Synthesis of gamma-carboxylated polypeptides by alpha-cells of the pancreatic islets

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Author

  • Leisa Stenberg
  • Elise Nilsson
  • Otto Ljungberg
  • Johan Stenflo
  • Mark A. Brown

Summary, in English

gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma -carboxylated zymogen that can be cleaved by ecarin to form an active serine protease that is inhibited by hirudin, The pancreas thus appears to be a novel site of synthesis for certain vitamin K-dependent proteins.

Publishing year

2001

Language

English

Pages

454-459

Publication/Series

Biochemical and Biophysical Research Communications

Volume

283

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • γ-carboxylation
  • Gla
  • pancreas
  • protein S
  • prothrombin
  • vitamin K

Status

Published

Research group

  • Clinical Chemistry, Malmö
  • Pathology, Malmö

ISBN/ISSN/Other

  • ISSN: 1090-2104