Poly(beta-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1
Author
Summary, in English
The moderate halophile Halomonas boliviensis, isolated from a Bolivian saline soil sample, was able to accumulate poly(P-hydroxybutyrate) (PHB) when grown under conditions of nutrient limitation and excess carbon source. The concentration of sodium chloride in the medium influenced the cell-growth, -size, and rate of PHB accumulation. Cultivation in shake flasks led to a PHB accumulation of about 54 wt.% with respect to cell dry weight at 4.5% (w/v) NaCl in a medium with butyric acid and sodium acetate as carbon sources. The production of PHB was substantially improved to a maximum value of 88 wt.% during cultivation under controlled conditions of pH and oxygen concentration in a fermentor. The use of glucose and sucrose, respectively, as carbon source could also lead to the production of PHB at an average level of 55 wt.%. (c) 2005 Elsevier Inc. All rights reserved.
Department/s
Publishing year
2006
Language
English
Pages
148-154
Publication/Series
Enzyme and Microbial Technology
Volume
38
Issue
1-2
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Industrial Biotechnology
Keywords
- carbon source
- poly(beta-hydroxybutyrate)
- halonionas boliviensis
- moderate halophile
- (PHB) accumulation
Status
Published
ISBN/ISSN/Other
- ISSN: 0141-0229