Enhanced Ribosome and tRNA Contents in Escherichia coli Expressing a Truncated Vitreoscilla Hemoglobin Mutant Analyzed by Flow Field-Flow Fractionation

Summary, in English

The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the mass fraction of active 70S ribosomes.

• Author: Charlotte Andersson; Cecilia Arfvidsson; P T Kallio; Karl-Gustav Wahlund; Leif Bülow
• Department/s: Pure and Applied Biochemistry; Centre for Analysis and Synthesis
• Publishing year: 2003
• Language: English
• Pages: 1499-1504
• Publication/Series: Biotechnology Letters
• Volume: 25
• Issue: 18
• Links: http://dx.doi.org/10.1023/A:1025475703006
• Document type: Journal article
• Publisher: Springer
• Topic: Biochemistry and Molecular Biology; Analytical Chemistry
• Status: Published
• ISBN/ISSN/Other: ISSN: 1573-6776