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miR-124 inhibits cell proliferation in breast cancer through downregulation of CDK4

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Author

  • Tongbao Feng
  • Dongqin Xu
  • Chao Tu
  • Wenjing Li
  • Yongling Ning
  • Jun Ding
  • Shizhong Wang
  • Liudi Yuan
  • Ning Xu
  • Keqing Qian
  • Yong Wang
  • Chunjian Qi
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Summary, in English

Studies have shown that microRNAs (miRNAs) are involved in the malignant progression of human cancer. However, little is known about the potential role of miRNAs in breast carcinogenesis. miR-124 expression in breast cancer tissue was measured by quantitative real-time PCR (qRT-PCR). Target prediction algorithms and luciferase reporter gene assays were used to investigate the target of miR-124. Breast cancer cells growth was regulated by overexpression or knockdown miR-124. At the end of the study, tumor-bearing mice were tested to confirm the function of miR-124 in breast cancer. In this study, we demonstrated that the expression of miR-124 was significantly downregulated in breast cancer tissues compared with matched adjacent non-neoplastic tissues. We identified and confirmed that cyclin-dependent kinase 4 (CDK4) was a direct target of miR-124. Overexpression of miR-124 suppressed CDK4 protein expression and attenuated cell viability, proliferation, and cell cycle progression in MCF-7 and MDA-MB-435S breast cancer cells in vitro. Overexpression of CDK4 partially rescued the inhibitory effect of miR-124 in the breast cancer cells. Moreover, we found that miR-124 overexpression effectively repressed tumor growth in xenograft animal experiments. Our results demonstrate that miR-124 functions as a growth-suppressive miRNA and plays an important role in inhibiting tumorigenesis by targeting CDK4.

Publishing year

2015

Language

English

Pages

5987-5997

Publication/Series

Tumor Biology

Volume

36

Issue

8

Document type

Journal article

Publisher

Springer

Topic

  • Cancer and Oncology

Keywords

  • Cell cycle
  • Proliferation
  • Breast cancer
  • CDK4
  • miR-124

Status

Published

ISBN/ISSN/Other

  • ISSN: 1423-0380