Acoustic Forces in Cytometry and Biomedical Applications: Multidimensional Acoustophoresis
Author
Summary, in English
Acoustophoresis, a method that utilizes acoustic forces to move particles and cells in microfluidic channels has been gaining increased attention over the last decade. The acoustophoretic method has been shown to handle a number of biosuspensions e.g., blood, cell cultures and raw milk as well as other biofluids, and comes with a variety of available unit operations e.g., free flow separation, binary density separation, particle positioning, contactless trapping, buffer changes, washing, and surface chemistry based sorting that allows integration into a wide range of application. The theoretical and experimental understanding of the acoustic radiation force which is the principal force used to manipulate particles in these systems (often generated with standing waves) has also evolved during this time. Chip-based acoustic systems have been presented in e.g., silicon, glass and PDMS, further illustrating the versatility of the method.
This dissertation presents some of the recent developments in the acoustophoretic field to illustrate how acoustic forces can be used in cytometry and biomedical applications, specifically by utilizing multiple acoustic wavelength geometries or two-dimensional particle manipulation. Paper I presents a novel way to pretreat raw milk in order to facilitate rapid quality control. Paper II extends this method by presenting a technique for label free cytometry in raw milk. Paper III showcases the ability to sort particles with fluorescence activated acoustic forces. Paper IV presents a low complexity high precision proof-of-concept sheathless impedance cytometer that can be integrated in other chip based systems. Paper V presents an improved method for concurrent blood component fractionation that requires less manual handling compared to established methods by implementing free flow separation into multiple outlets.
The theory section explains the underlying physical laws that govern the microscale fluid systems presented here. Acoustic force theory is explained in detail for better understanding of the acoustic radiation forces that act on the suspended particles and also cause media streaming. The particle manipulation section compares the different methods that are available to researchers in the biomedical microfluidic field. The microfabrication section deals with the design aspects of using various materials. Unit operations and applications specific for acoustophoresis are presented. Biofluids and cell types including blood and raw milk are discussed to underline the challenges that researchers are faced with during system design, handling and analysis. The aim of this dissertation is to provide a foundation for future development of acoustic force applications in cytometry and biomedicine.
Department/s
Publishing year
2014
Language
English
Publication/Series
ISRN: LUTEDX/TEEM – 1096 – SE Report: 3/14
Full text
- Available as PDF - 21 MB
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Document type
Dissertation
Publisher
Department of Biomedical Engineering, Lund university
Topic
- Medical Engineering
Keywords
- Acoustophoresis
- Microfluidics
- Ultrasound
- Multinode
- Cell Sorting
- Particle Positioning
- Cytometry
- Lab-on-a-Chip
- µTAS
- Binary Separation
- Acoustic Barriers
- Acoustic Particle Manipulation
- Acoustic Standing Waves
- Blood Separation
- Milk Analysis
Status
Published
Supervisor
ISBN/ISSN/Other
- ISBN: 978-91-7623-194-4 (print)
- ISBN: 978-91-7623-195-1 (pdf)
Defence date
5 December 2014
Defence time
10:15
Defence place
Lecture hall E:1406, E-building, Ole Römers väg 3, Lund University, Faculty of Engineering, LTH
Opponent
- Steven Graves (Dr, Assoc. Prof.)