Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.

Summary, in English

The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.

• Author: Aleksandra Dabkowska; Gaelle Piret; Cassandra Niman; Mercy Lard; Heiner Linke; Tommy Nylander; Christelle Prinz
• Department/s: Physical Chemistry; NanoLund: Centre for Nanoscience; Solid State Physics; Neuronano Research Center (NRC)
• Publishing year: 2015
• Language: English
• Pages: 18020-18024
• Publication/Series: Nanoscale
• Volume: 7
• Issue: 43
• Full text: - 1 MB; Download statistics
• Links: http://dx.doi.org/10.1039/c5nr05427c
• Document type: Journal article
• Publisher: Royal Society of Chemistry
• Topic: Biophysics
• Status: Published
• Research group: Neuronano Research Center (NRC)
• ISBN/ISSN/Other: ISSN: 2040-3372