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Quantitative Label-Free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family

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Author

  • Svante Resjo
  • Ashfaq Ali
  • Harold J. G. Meijer
  • Michael F. Seidl
  • Berend Snel
  • Marianne Sandin
  • Fredrik Levander
  • Francine Govers
  • Erik Andreasson

Summary, in English

The oomycete Phytophthora infestans is the causal agent of late blight in potato and tomato. Since the underlying processes that govern pathogenicity and development in P. infestans are largely unknown, we have performed a large-scale phosphoproteomics study of six different P. infestans life stages. We have obtained quantitative data for 2922 phosphopeptides and compared their abundance. Life-stages-pecific phosphopeptides include ATP-binding cassette transporters and a kinase that only occurs in appressoria. In an extended data set, we identified 2179 phosphorylation sites and deduced 22 phosphomotifs. Several of the phosphomotifs matched consensus sequences of kinases that occur in P. infestans but not Arabidopsis. In addition, we detected tyrosine phosphopeptides that are potential targets of kinases resembling mammalian tyrosine kinases. Among the phosphorylated proteins are members of the RXLR and Crinkler effector families. The latter are phosphorylated in several life stages and at multiple positions, in sites that are conserved between different members of the Crinkler family. This indicates that proteins in the Crinkler family have functions beyond their putative role as (necrosis-inducing) effectors. This phosphoproteomics data will be instrumental for studies on oomycetes and host oomycete interactions. The data sets have been deposited to ProteomeXchange (identifier PXD000433).

Publishing year

2014

Language

English

Pages

1848-1859

Publication/Series

Journal of Proteome Research

Volume

13

Issue

4

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Immunology in the medical area

Keywords

  • Phytophthora infestans
  • late blight
  • potato
  • protein phosphorylation
  • proteomics
  • phosphoproteomics
  • effectors
  • Crinklers
  • CRN proteins
  • tyrosine phosphorylation
  • phosphorylation motifs
  • appressorium

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-3893