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Role of group A streptococcal IgG-binding proteins in triggering experimental glomerulonephritis in the rabbit

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Author

  • L Burova
  • A Thern
  • P Pigarevsky
  • M Gladilina
  • V Seliverstova
  • E Gavrilova
  • V Nagornev
  • Claës Schalén
  • A Totolian

Summary, in English

Our previous studies have indicated that the IgG-binding M-family proteins (IgGBP) of group A streptococci may be involved in eliciting experimental acute poststreptococcal glomerulonephritis (APSGN) in the rabbit. These surface proteins were also found to trigger production of anti-IgG, which might conceivably act to enhance renal deposition of immune complexes (IC). In the present study, a clinical isolate of serotype M22 (strain AL168), an isogenic double mutant deficient for both the IgGBPs Mrp and Emm, as well as mutants deficient in only one of the proteins were tested for capacity to induce glomerulonephritis. Streptococci to be used for injecting rabbits were heat-killed. Surface-bound IgG was removed by 1 M KSCN and cells were then repeatedly washed in PBS before use. Rabbits were injected intravenously with 10(9) cells three times a week for 8 weeks and, following one month of rest, for another 6 weeks. Deposits of IgG and C3 as well as induced chemokines TNF-alpha, IL-1beta and IL-6 were traced in cryostat sections using specific antibodies and appropriate peroxidase-labelled anti-antibodies. In four rabbits immunized with the double mutant strain, no deposits were found, and as examined by TEM, only subtle and transient renal changes were observed. In contrast, the original strain AL168 induced pronounced inflammatory and degenerative glomerular changes in all four rabbits injected, and deposits of TNF-alpha, IL-1beta and IL-6 were found in mesangial and endothelial cells. Similar deposits and glomerular changes were seen in all eight rabbits injected with the mrp-emm+ mutant and in four out of seven animals receiving the mrp+emm- mutant. There was a highly significant correlation between high levels of circulating anti-IgG and development of APSGN. These results confirm an important role of streptococcal IgGBP in triggering experimental APSGN as earlier proposed by our group.

Publishing year

2003

Language

English

Pages

955-962

Publication/Series

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Volume

111

Issue

10

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Microbiology in the medical area

Keywords

  • Streptococcus pyogenes
  • poststreptococcal glomerulonephritis
  • rabbit
  • model

Status

Published

ISBN/ISSN/Other

  • ISSN: 1600-0463