Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana
Author
Summary, in English
Iron uptake in Arabidopsis thaliana is mediated by ferric chelate reductase FRO2, a transmembrane protein belonging to the flavocytochrome b family. There is no high resolution structural information available for any member of this family. We have determined the transmembrane topology of FRO2 experimentally using the alkaline phosphatase fusion method. The resulting topology is different from that obtained by theoretical predictions and contains 8 transmembrane helices, 4 of which build up the highly conserved core of the protein. This core is present in the entire flavocytochrome b family. The large water soluble domain of FRO2, which contains NADPH, FAD and oxidoreductase sequence motifs, was located on the inside of the membrane.
Department/s
Publishing year
2006
Language
English
Pages
215-221
Publication/Series
Plant Molecular Biology
Volume
62
Issue
1-2
Document type
Journal article
Publisher
Springer
Topic
- Biological Sciences
Keywords
- gp91phox
- FRO2
- ferric-chelate reductases
- oxidases
- superoxide generating NADPH
- iron transport
- alkaline phosphatase
Status
Published
ISBN/ISSN/Other
- ISSN: 1573-5028