Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model
Author
Summary, in English
To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.
Publishing year
1991
Language
English
Pages
379-383
Publication/Series
Acta Oncologica
Volume
30
Issue
3
Document type
Journal article
Publisher
Taylor & Francis
Topic
- Cancer and Oncology
Keywords
- Monoclonal antibodies
- colon carcinoma
- rat
Status
Published
Research group
- Medical Radiation Physics, Malmö
ISBN/ISSN/Other
- ISSN: 1651-226X