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A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

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Author

Summary, in English

Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (similar to 2 nm to 50 mu m in diameter) and molecular weight range (10(3)-10(10) g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets. (C) 2013 Elsevier B.V. All rights reserved.

Department/s

  • Department of Food Technology, Engineering and Nutrition

Publishing year

2014

Language

English

Pages

25-33

Publication/Series

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Volume

442

Document type

Journal article

Publisher

Elsevier

Topic

  • Food Engineering

Keywords

  • Field-flow fractionation
  • Colloids
  • Proteins
  • Beta-glucans
  • Aggregates
  • Emulsions

Status

Published

ISBN/ISSN/Other

  • ISSN: 0927-7757