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Gender and strain influence on neurogenesis in dentate gyrus of young rats

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Author

  • Ekaterina Perfilieva
  • Anette Risedal
  • Jenny Nyberg
  • Barbro Johansson
  • Peter S Eriksson

Summary, in English

To investigate whether rat hippocampal neurogenesis varies with strain and gender, the authors examined proliferating progenitor cells and their progeny in young male and female Sprague-Dawley (SD) and spontaneously hypertensive rats (SHR) using the thymidine analog bromodeoxyuridine (BrdU) combined with immunohistochemistry for the neuronal marker Calbindin D28k and glial fibrillary acidic protein. Rats were given 7 consecutive daily BrdU injections and were killed 1 day or 4 weeks later to allow for discrimination between proliferation and cell survival. Stereologic analysis of the numbers of BrdU-immunoreactive cells in the dentate gyrus revealed both a strain difference with significantly higher cell proliferation and net neurogenesis in SHR than in SD and a gender difference with males from both strains producing significantly more cells than their female counterparts. Whereas the number of progenitors four weeks after BrdU injections was still significantly greater in male than in female SHRs, resulting in a greater net neurogenesis in the male, the number of BrdU-immunoreactive cells did not differ between male and female SD rats, suggesting a greater survival of newly generated cells in the dentate gyrus in female than in male SD rats. No sex or strain difference was observed in the relative ratio of neurogenesis and gliogenesis.

Department/s

Publishing year

2001

Language

English

Pages

211-217

Publication/Series

Journal of Cerebral Blood Flow and Metabolism

Volume

21

Issue

3

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Cardiac and Cardiovascular Systems

Keywords

  • Neurogenesis
  • Progenitor cells
  • Gender difference
  • Strain difference
  • SHR
  • Sprague-Dawley

Status

Published

ISBN/ISSN/Other

  • ISSN: 1559-7016