Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase
Author
Summary, in English
The cDNAs encoding human prostatic acid phosphatase were cloned and characterized. The mRNAs contain 3' noncoding regions of heterogeneous sizes 646, 1887 or 1913 nucleotides. A dimer and a monomer of the conserved Alu-repeats are present in the longer 3' noncoding sequences. The complete sequence of 354 amino acids for the mature enzyme was determined by sequencing both cDNA and protein. Human prostatic and lysosomal acid phosphatases exhibit 50% sequence homology, including five Cys residues and two putative N-linked glycosylation sites. The Acp-3 gene coding for human prostatic acid phosphatase was mapped onto chromosome 3 in this investigation. The Acp-2 gene coding for lysosomal acid phosphatase has previously been located on chromosome 11, while the Acp-1 gene coding for red blood cell acid phosphatase is on chromosome 2.
Publishing year
1989
Language
English
Pages
79-86
Publication/Series
Biochem Biophys Res Commun
Volume
160
Issue
1
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Medicinal Chemistry
Keywords
- Macromolecular Substances
- Lysosomes/*enzymology
- Humans
- Glycosylation
- DNA/*genetics
- Comparative Study
- Molecular
- *Cloning
- Pair 3
- Human
- Chromosomes
- *Chromosome Mapping
- Base Sequence
- Acid Phosphatase/*genetics
- Amino Acid Sequence
- Male
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Prostate/*enzymology
- RNA
- Messenger/genetics
- Sequence Homology
- Nucleic Acid
Status
Published