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Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium

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Author

Summary, in English

The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes.

Publishing year

2003

Language

English

Pages

271-277

Publication/Series

Molecular Human Reproduction

Volume

9

Issue

5

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Obstetrics, Gynecology and Reproductive Medicine

Keywords

  • proteinase
  • mRNA
  • MMP-26
  • c-jun
  • human endometrium
  • regulation

Status

Published

ISBN/ISSN/Other

  • ISSN: 1460-2407