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Thrombin-mediated proteolysis of factor V resulting in gradual B-domain release and exposure of the factor Xa-binding site.

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Author

Summary, in English

To investigate the relationship between the individual thrombin-cleavages in factor V (FV) and the generation of FXa-cofactor activity, recombinant FV mutants having the cleavage sites eliminated separately or in combination were used. After thrombin incubation, the ability of the FV variants to bind FXa and support prothrombin activation was tested. The interaction between FVa and FXa on the surface of phospholipid was investigated with a direct binding assay as well as in a functional prothrombin-activation assay. FV mutated at all cleavage sites functioned poorly as FXa cofactor in prothrombin activation, the apparent Kd for FXa being approximately 10 nM. Fully activated wt-FVa, yielded an apparent Kd of around 0.2 nM. The Arg709 and Arg1018 cleavages occurred at low thrombin concentrations, and decreased the Kd for FXa binding 5- and 3-fold, respectively. The Arg1545 cleavage, being less sensitive to thrombin, decreased the Kd for FXa binding approximately 20-fold. The Km for prothrombin was the same for all FV variants, demonstrating B-domain dissociation to result in exposure of binding site for FXa, but not for prothrombin. In conclusion, we demonstrate FV activation to be associated with the stepwise release of the B-domain, which results in a gradual exposure of the FXa-binding site.

Publishing year

2002

Language

English

Pages

38424-38430

Publication/Series

Journal of Biological Chemistry

Volume

277

Issue

41

Document type

Journal article

Publisher

American Society for Biochemistry and Molecular Biology

Topic

  • Medicinal Chemistry

Status

Published

Research group

  • Clinical Chemistry, Malmö

ISBN/ISSN/Other

  • ISSN: 1083-351X