Improved preparation technique of cervical carcinoma for flow cytometric DNA analysis with tissue disintegration in hydrochloric acid
Author
Summary, in English
An one-step procedure using a nuclear isolation medium containing propidium iodide has been found to be a suitable preparation technique for flow cytometric DNA analysis in breast cancer samples. In the case of cervical squamous carcinoma, a pretreatment with HCl seems to be a methodological improvement. One advantage with the HCl modification is that some "false" near-diploid cell populations are abolished. These "false" G0/G1 peaks may represent diploid nuclei with a different stainability for propidium iodide compared to normal diploid nuclei. The HCl treatment has, furthermore, the advantage of increasing the elution of nuclei (mean factor of 4.0), especially non-diploid nuclei from higher differentiated squamous carcinomas.
Department/s
Publishing year
1990
Language
English
Pages
225-229
Publication/Series
In Vivo
Volume
4
Issue
4
Document type
Journal article
Publisher
In vivo
Topic
- Cancer and Oncology
Status
Published
ISBN/ISSN/Other
- ISSN: 0258-851X