Modeling pathophysiological aspects of Parkinson’s disease: Manipulating DA handling and alpha-synuclein expression in the nigrostriatal pathway using viral vectors
Author
Summary, in English
The pathological hallmark of Parkinson’s disease is dopaminergic neurodegeneration in the substantia nigra
pars compacta neurons and accumulation of α-synuclein containing aggregates in the surviving neurons. The
cause of cell death in Parkinson’s disease and the involvement of α-synuclein in the pathophysiology of the
disease are unknown. Although other neuronal cell types exhibit α-synuclein positive aggregates, substantia
nigra dopamine neurons display a selective vulnerability to α-synuclein mediated neurodegeneration. In this
thesis work I have focused on the possible mechanisms underlying the vulnerability of dopamine producing
neurons against α-synuclein induced neurotoxicity. To study the molecular interactions playing role in
α-synuclein mediated dopaminergic neurodegeneration, we investigated putative mechanisms that has been
implicated in α-synuclein toxicity. Interaction of the α-synuclein protein with other molecules has been
suggested to enhance the aggregation. We studied the interaction between the full-length α-synculein protein
and truncated α-synuclein in the rat substantia nigra. When the two forms are co-expressed the truncated form
promotes full-length α-syn aggregation and enhance the pathology caused by the full-length protein. We next
investigated the specific role of dopamine handling machinery in Parkinson’s disease pathophysiology and
treatment related motor complications. To study the involvement of cytosolic DA and age related increase in
the reactive DA species on α-syn toxicity, we utilized a transgenic mouse model carrying a hypomorphic
VMAT2 mutation. The elevated cytosolic dopamine in these mice led to an increased vulnerability to
α-synuclein overexpression. To show that this vulnerability was indeed dopamine dependent, we generated
recombinant adeno-associated viral vectors to transfer short hairpin RNA sequences targeting the
rate-limiting enzyme, tyrosine hydroxylase. Reducing dopamine production using the shRNA approach in
these mice rescued the vulnerability against α-synuclein in the nigral dopamine neurons. Our results implicate
the critical role of dopamine handling in Parkinson’s disease pathophysiology, thus suggest that regulating
the specific pathways through which DA mediates its toxic effects can prevent the potential
neurodegeneration.
pars compacta neurons and accumulation of α-synuclein containing aggregates in the surviving neurons. The
cause of cell death in Parkinson’s disease and the involvement of α-synuclein in the pathophysiology of the
disease are unknown. Although other neuronal cell types exhibit α-synuclein positive aggregates, substantia
nigra dopamine neurons display a selective vulnerability to α-synuclein mediated neurodegeneration. In this
thesis work I have focused on the possible mechanisms underlying the vulnerability of dopamine producing
neurons against α-synuclein induced neurotoxicity. To study the molecular interactions playing role in
α-synuclein mediated dopaminergic neurodegeneration, we investigated putative mechanisms that has been
implicated in α-synuclein toxicity. Interaction of the α-synuclein protein with other molecules has been
suggested to enhance the aggregation. We studied the interaction between the full-length α-synculein protein
and truncated α-synuclein in the rat substantia nigra. When the two forms are co-expressed the truncated form
promotes full-length α-syn aggregation and enhance the pathology caused by the full-length protein. We next
investigated the specific role of dopamine handling machinery in Parkinson’s disease pathophysiology and
treatment related motor complications. To study the involvement of cytosolic DA and age related increase in
the reactive DA species on α-syn toxicity, we utilized a transgenic mouse model carrying a hypomorphic
VMAT2 mutation. The elevated cytosolic dopamine in these mice led to an increased vulnerability to
α-synuclein overexpression. To show that this vulnerability was indeed dopamine dependent, we generated
recombinant adeno-associated viral vectors to transfer short hairpin RNA sequences targeting the
rate-limiting enzyme, tyrosine hydroxylase. Reducing dopamine production using the shRNA approach in
these mice rescued the vulnerability against α-synuclein in the nigral dopamine neurons. Our results implicate
the critical role of dopamine handling in Parkinson’s disease pathophysiology, thus suggest that regulating
the specific pathways through which DA mediates its toxic effects can prevent the potential
neurodegeneration.
Department/s
Publishing year
2010
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2010:61
Document type
Dissertation
Publisher
Department of Experimental Medical Science, Lund Univeristy
Topic
- Neurosciences
Keywords
- Parkinson's disease
- adeno-associated virus
- dopamine
- α-synuclein
- tyrosine hydroxylase
- RNAinterference
- shRNA
- substantia nigra
- animal models
- dyskinesia
Status
Published
Research group
- Brain Repair and Imaging in Neural Systems (BRAINS)
Supervisor
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-86443-76-4
Defence date
22 May 2010
Defence time
09:15
Defence place
Segerfalk Lecture Hall
Opponent
- Piers C Emson