Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

Summary, in English

Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.

• Author: Lena Stenson; Anna Maria Hoffmann; Hindrik Mulder; Frank Sundler; Cecilia Holm; Anders Bergh; Gudrun Fredrikson
• Department/s: Insulin Signal Transduction; Department of Experimental Medical Science; Molecular Endocrinology
• Publishing year: 1994
• Language: English
• Pages: 125-130
• Publication/Series: FEBS Letters
• Volume: 355
• Issue: 2
• Links: http://dx.doi.org/10.1016/0014-5793(94)01185-0
• Document type: Journal article
• Publisher: Wiley-Blackwell
• Topic: Biological Sciences
• Keywords: Hormone sensitive lipase; Cholesterol ester hydrolase; Testis; Sertoli cell; In situ hybridization
• Status: Published
• Research group: Insulin Signal Transduction; Molecular Endocrinology
• ISBN/ISSN/Other: ISSN: 1873-3468