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The Lecticans of Mammalian Brain Perineural Net Are O-Mannosylated

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Author

  • Sandra Pacharra
  • Franz-Georg Hanisch
  • Martina Muehlenhoff
  • Andreas Faissner
  • Uwe Rauch
  • Isabelle Breloy

Summary, in English

O-Mannosylation is an important protein modification in brain. During the last years, a few mammalian proteins have been identified as targets of the protein-O-mannosyltransferases 1 and 2. However, these still cannot explain the high content of O-mannosyl glycans in brain and the strong brain involvement of congenital muscular dystrophies caused by POMT mutations (Walker-Warburg syndrome, dystroglycanopathies). By fractionating and analyzing the glycoproteome of mouse and calf brain lysates, we could show that proteins of the perineural net, the lecticans, are O-mannosylated, indicating that major components of neuronal extracellular matrix are O-mannosylated in mammalian brain. This finding corresponds with the high content of O-mannosyl glycans in brain as well as with the brain involvement of dystroglycanopathies. In contrast, the lectican neurocan is not O-mannosylated when recombinantly expressed in EBNA-293 cells, revealing the possibility of different control mechanisms for the initiation of O-mannosylation in different cell types.

Publishing year

2013

Language

English

Pages

1764-1771

Publication/Series

Journal of Proteome Research

Volume

12

Issue

4

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Cell and Molecular Biology

Keywords

  • O-glycans
  • O-mannosylation
  • lecticans
  • ECM
  • perineural net
  • O-glycosylation
  • ESI-MS/MS
  • MALDI-MS/MS
  • glycoproteomics
  • dystroglycanopathies

Status

Published

Research group

  • Vessel Wall Biology

ISBN/ISSN/Other

  • ISSN: 1535-3893