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Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density

På svenska

Author

  • Nikolay V. Berezhnoy
  • Dan Lundberg
  • Nikolay Korolev
  • Chenning Lu
  • Jiang Yan
  • Maria Miguel
  • Björn Lindman
  • Lars Nordenskioeld

Summary, in English

In this work we have investigated the structures of aggregates formed in model systems of dilute aqueous mixtures of "model chromatin" consisting of either recombinant nucleosome core particles (NCPs) or nucleosome arrays consisting of 12 NCPs connected with 30 bp linker DNA, and liposomes made from different mixtures of cationic and zwitterionic lipids, 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The aggregates formed were characterized using different optical microscopy methods and small-angle X-ray scattering (SAXS), and the results are discussed in terms of the competing intermolecular interactions among the components. For a majority of the samples, the presence of lamellar structures could be identified. Ir. samples with high fractions of DOTAP in the liposomes, well-defined lamellar structures very similar to those formed by the corresponding lipid mixtures and DNA alone (i.e., without histone proteins) were observed; in these aggregates, the histones are expelled from the model chromatin. The findings suggest that, with liposomes containing large fractions of cationic lipid, the dominating driving force for aggregation is the increase in translational entropy from the release of counterions, whereas with lower fractions of the cationic lipid, the entropy of mixing of the lipids within the bilayers results in a decreased DNA-lipid attraction.

Department/s

Publishing year

2012

Language

English

Pages

4146-4157

Publication/Series

Biomacromolecules

Volume

13

Issue

12

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Physical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1526-4602