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Tenascins are associated with lipid rafts isolated from mouse brain

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Author

  • J Kappler
  • SL Baader
  • S Franken
  • P Pesheva
  • K Schilling
  • Uwe Rauch
  • V Gieselmann

Summary, in English

Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts. (C) 2002 Elsevier Science (USA). All rights reserved.

Publishing year

2002

Language

English

Pages

742-747

Publication/Series

Biochemical and Biophysical Research Communications

Volume

294

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • cerebellum
  • tenascin
  • lipid rafts

Status

Published

Research group

  • Vessel Wall Biology

ISBN/ISSN/Other

  • ISSN: 1090-2104