Comparison of NCL-hTERT antibody reactivity and telomere repeat amplification protocol in situ in effusions
Author
Summary, in English
Objective To compare the performances of 2 methods, telomerase repeat amplification protocol (TRAP) in situ and antibodies to the hTERT protein, in assessing telomerase activity. Study Design TRAP in situ and immunomercial antibody (NCL-hTERT) was performed on 54 body cavity effusions. The results were compared and correlated to diagnosis. Results Thirty-four effitsions from patients with verified malignant disease contained cytologically malignant cells. Both methods were positive in 33 of the cases, whereas only hTERT was positive in I case. Twenty effusions, all containing mesothelial cells, came from patients with benign conditions. In 2 fluids atypical, hyperplastic mesothelial cells were both TRAP in situ and hTERT positive. All remaining 18 fluids were TRAP in situ negative, whereas 12 of 18 were hTERT positive. Thus the results of TRAP in situ and hTERT immunohistochemistry disagreed in 1 of 34 (3%) malignant and 12 of 20 (60%) benign cases. Conclusion The sensitivities for malignancy were similar for TRAP in situ and hTERT immunobistochemistry. The specificity of the applied hTERT antibody was significantly lower, due to hTERT reactivity in mesothelial cells.
Department/s
Publishing year
2007
Language
English
Pages
886-892
Publication/Series
Acta Cytologica
Volume
51
Issue
6
Links
Document type
Journal article
Publisher
Karger
Topic
- Cancer and Oncology
- Cell and Molecular Biology
Keywords
- nucleolin
- mesothelial cells
- malignancy
- immunohistochemistry
- effusion
- hTERT
- telomere repeat amplification protocol
- telomerase
Status
Published
Research group
- Pathology, Malmö
ISBN/ISSN/Other
- ISSN: 0001-5547