Expression and function of the protein tyrosine phosphatases SHP-1 and SHP-2 in prostate cancer
Author
Summary, in English
We performed several different in vitro and in vivo experiments that were evaluated with methods such as immunohistochemical staining of tissue micro arrays (TMA), polymerase chain reaction (PCR), Western blot, eukaryotic cell transfections, proliferation assays, PTP enzymatic activity assay, caspase assay, flow cytometric assays, cell growth in vivo in nude mice, migration and invasion assays.
Our results show that a low or absent SHP-1 expression might serve as a marker to identify prostate cancer patients at an increased risk of tumour recurrence. We also demonstrate that proliferation and apoptosis can be regulated by modulations of the SHP-1 expression in prostate cancer cells. SHP-1 expression and activity is reduced in the LNCaP cell line that was long-term stimulated with IL-6, showing that the cytokine might regulate SHP-1 activity. SHP-1 is also able to reduce growth of PC3 cells growing in vivo in nude mice. These results suggest that SHP-1 is a negative modulator by inhibiting prostate cancer cell proliferation through the growth factor and cytokine-induced pathways in prostate cancer cells. Our results also suggest that SHP-2 may have similar activity in prostate cancer.
Department/s
Publishing year
2010
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2010:24
Document type
Dissertation
Publisher
Lund University: Faculty of Medicine
Topic
- Cancer and Oncology
Keywords
- Prostate cancer SHP-1SHP-2 IL-6phosphorylationsignalling pathways
Status
Published
Supervisor
- Anette Gjörloff Wingren
- Pirkko Härkönen
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-86443-38-2
Defence date
26 March 2010
Defence time
09:00
Defence place
MFC entrance 59, Malmö University Hospital
Opponent
- Pernilla Wikström (docent)