The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination

På svenska

Author

Summary, in English

Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization. procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyarnines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma.

Publishing year

2008

Language

English

Pages

184-193

Publication/Series

Analytica Chimica Acta

Volume

606

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Analytical Chemistry

Keywords

  • polyamines
  • extraction technique
  • hollow fiber membrane
  • supported liquid membrane

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4324