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Plasma enterostatin: Identification and release in rats in response to a meal

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Author

Summary, in English

Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with I-125-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma. Discussion: The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake.

Publishing year

2002

Language

English

Pages

688-694

Publication/Series

Obesity Research

Volume

10

Issue

7

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Other Clinical Medicine
  • Nutrition and Dietetics

Keywords

  • fat intake
  • procolipase
  • appetite
  • sucrose
  • enzyme-linked immunosorbent
  • assay

Status

Published

Research group

  • Appetite Regulation

ISBN/ISSN/Other

  • ISSN: 1071-7323