FACS binding assay for analysing GDNF interactions.

Summary, in English

Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cells allowed readily detection of GDNF binding and displacement to endogenous receptors. The dissociation constant and half maximal inhibitory concentration obtained were comparable to other studies using standard binding assays. Overall, this FACS-based, simple to perform and adaptable to high throughput setup, provides a safer and reliable alternative to radioactive methods.

• Author: Luis Quintino; Aurelie Baudet; Jonas Larsson; Cecilia Lundberg
• Department/s: CNS Gene Therapy; Division of Molecular Medicine and Gene Therapy; StemTherapy: National Initiative on Stem Cells for Regenerative Therapy; MultiPark: Multidisciplinary research focused on Parkinson´s disease
• Publishing year: 2013
• Language: English
• Pages: 25-28
• Publication/Series: Journal of Neuroscience Methods
• Volume: 218
• Issue: 1
• Links: http://www.ncbi.nlm.nih.gov/pubmed/23669067?dopt=Abstract; http://dx.doi.org/10.1016/j.jneumeth.2013.04.006
• Document type: Journal article
• Publisher: Elsevier
• Topic: Neurosciences
• Status: Published
• Research group: CNS Gene Therapy
• ISBN/ISSN/Other: ISSN: 1872-678X