The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutein.

På svenska

Author

Summary, in English

Flow field-flow fractionation (FFF) has previously been used in successful fractionation and characterisation of the ultra-large wheat protein glutenin. The many parameters, which may influence the retention behaviour, especially when analysing extremely high-molecular-mass samples such as glutenin, are here reported. Size determination from the sample retention time, using FFF theory, will as a result have a very low accuracy. The need for direct molecular mass determination, such as by light scattering, in combination with FFF, in order to do accurate size measurements of glutenin is pointed out as well as the importance to minimise the overloading.

Publishing year

2003

Language

English

Pages

99-109

Publication/Series

Journal of Chromatography A

Volume

1011

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Analytical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 0021-9673