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Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein

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Author

Summary, in English

Freshly ejaculated human semen has the appearance of a loose gel in which the predominant structural protein components are the seminal vesicle-secreted semenogelins (Sg). The primary structure of the 439-residue SgI has previously been obtained by cDNA cloning. This cDNA cross-hybridizes to a larger transcript coding for a second secretory protein, SgII. Here we report the almost complete structure of a precursor of SgII established by lambda gt11 clones isolated from epididymal and seminal vesicular cDNA libraries. The deduced amino acid sequence of the 559-residue mature protein has a molecular weight of 62,931 but an increase in weight may be provided by asparagine-linked oligosaccharide attachment at residue 249. SgII, which has 78% overall identity with SgI, contains eight 60-residue regions that display conspicuous internal sequence similarity, whereas SgI only contains six of these regions. The SgII structure is translated from an open reading frame in a polyadenylylated 2.4-kilobase transcript. The message is abundant in the seminal vesicles but rare in the epididymis.

Publishing year

1992

Language

English

Pages

63-4559

Publication/Series

Proc Natl Acad Sci U S A

Volume

89

Issue

10

Document type

Journal article

Topic

  • Medicinal Chemistry

Keywords

  • Research Support
  • Protein Precursors/*genetics
  • Oligodeoxyribonucleotides
  • Molecular Weight
  • Molecular Sequence Data
  • Male
  • Humans
  • Gonadal Steroid Hormones/*genetics
  • Gene Library
  • Epididymis/*physiology
  • DNA/genetics/isolation & purification
  • Comparative Study
  • Molecular/methods
  • Cloning
  • Northern
  • Blotting
  • Amino Acid Sequence
  • Base Sequence
  • Non-U.S. Gov't
  • Restriction Mapping
  • Semen/*physiology
  • *Seminal Plasma Proteins
  • *Seminal Vesicle Secretory Proteins
  • Seminal Vesicles/*physiology
  • Sequence Homology
  • Nucleic Acid
  • *Transcription
  • Genetic

Status

Published

Research group

  • Clinical Chemistry, Malmö