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Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.

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Author

  • Hae Jong Kim
  • Julhash U. Kazi
  • You Ree Lee
  • Dung H. Nguyen
  • Hyang Bok Lee
  • Jeong Hyun Shin
  • Jae Won Soh
  • Eun Ki Kim

Summary, in English

Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer.

Publishing year

2010-02-01

Language

English

Pages

249-253

Publication/Series

International Journal of Molecular Medicine

Volume

25

Issue

2

Document type

Journal article

Publisher

Spandidos Publications

Status

Published

ISBN/ISSN/Other

  • ISSN: 1791-244X