Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp nov.

Summary, in English

Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA-DNA reassociation, and phenotypically by using fatty acid methyll ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of water-borne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962(T) (= B2156(T) = CIP 106911(T) = NCIMB 13772(T)).

• Author: NA Logan; G Forsyth; L Lebbe; J Goris; M Heyndrickx; A Balcaen; A Verhelst; E Falsen; Åsa Ljungh; HB Hansson; P De Vos
• Department/s: Division of Medical Microbiology
• Publishing year: 2002
• Language: English
• Pages: 953-966
• Publication/Series: International Journal of Systematic and Evolutionary Microbiology
• Volume: 52
• Links: http://dx.doi.org/10.1099/ijs.0.02081-0
• Document type: Journal article
• Publisher: Microbiology Society
• Topic: Microbiology
• Keywords: Brevibacillus agri waterborne illness; Bacillus badius; Aneurinibacillus; Brevibacillus; invocatus
• Status: Published
• ISBN/ISSN/Other: ISSN: 1466-5026